00 for referral to fertility clinic), or drug prescriptions used exclusively to treat fertility AUY-922 in vivo problems in women (principally clomiphene citrate).24 We considered the date of the first record of a fertility problem during the study period to be the date of a new clinically recorded fertility problem. A detailed description of how we defined incident records of fertility problems is available elsewhere.19 This

definition of new clinically recorded fertility problems was shown in our previous work to generate age-specific rates with comparable patterns with those reported by the Human Fertilisation and Embryology Authority, which reports population-based, age-specific rates of women receiving specialized fertility treatments in the United Kingdom.25 Code lists are available Epigenetics inhibitor from the authors upon request. Information on women’s sociodemographic factors including age, socioeconomic status, as measured by quintiles of the Townsend Deprivation Index, the most recent smoking status record, and body mass index (BMI) before the first fertility problem record was extracted. For women who did not have a recorded fertility problem, a random date was generated

(pseudodiagnosis date) as a reference to extract the most recent recording on smoking status and BMI. Women were classified as smokers and nonsmokers (including never smokers and ex-smokers). If the medical code did not clearly indicate whether women were smokers or not, they were included in the missing/unknown category. Information on BMI was categorized as follows: underweight (<18.5 Rutecarpine kg/m2), normal (18.5–24.9 kg/m2), overweight (25.0–29.9 kg/m2), obese (≥30 kg/m2), and missing BMI. Information on other autoimmune disorders including type 1 diabetes, rheumatoid arthritis, and thyroid disorders also was extracted. We described and compared baseline characteristics among women with and without CD using means, t tests, proportions, and chi-square tests. The

distribution of all types of fertility problems across the study period was examined in both women with CD and women without CD. We estimated the incident rates of new clinically recorded fertility problems as the number of first recorded fertility problem per 1000 person-years. Female fertility is known to decrease with age 26 and 27; therefore, we stratified the rates of clinically recorded fertility problems by 5-year age groups. We used lexis expansion 28 to construct an age-cohort model in which women could contribute person time to more than one age group. Given that the prevalence of CD has increased over time 29 we used an additional lexis expansion to split the study time by calendar year. We calculated age-specific incident rates of clinically recorded fertility problems in women with CD compared with women without CD.