2 μM to 1.1 mM) [14]. The excellent performance may be attributed to the possible synergetic effect between Pt and Cu [15] and the porous structure of the PtCu NCs, which provide

a large specific surface area. In terms of the synergetic effect, Cu atom in the PtCu alloy acts both as promoting centers for the generation of the Cu-OHad species and as an electron donor to Pt in the PtCu alloy. The incorporation of Cu atom learn more decreases the Pt 4f binding energies GSK458 ic50 and consequently reduces the Pt-OHad bond strength. Therefore, the intimate contact between Pt and Cu domains in the PtCu alloy greatly promotes the regeneration of Pt sites for high electrochemical activity towards hydrogen peroxide. Figure 3 Current-time response of PtCu NC electrode towards H 2 O 2 . The inset shows

the relationship between the catalytic current and the concentration of H2O2. To estimate the effective surface area of the PtCu NC this website electrode, cyclic voltammograms on PtCu NC electrode in a solution containing 5 mM K3Fe(CN)6 and 0.1 M KCl were performed [16]. According to the Randles-Sevcik equation [17], (4) where A is the effective surface area (cm2), I p is the peak current of the redox reaction of [Fe(CN)6]3-/4- (A), n is the number of electrons transferred (n = 1), D is the diffusion coefficient (0.76 × 10-5 cm2 s-1), v is the scan rate (V s-1), and C is the concentration of K3Fe(CN)6 (5 mM). The calculated value of A is 0.83 cm2 for the PtCu NC electrode, which is 11.75 times of the bare GCE. Conclusions Cubic PtCu NCs were successfully synthesized using Cu2O as the template. The PtCu NC electrode exhibited excellent electrocatalytic activity towards H2O2. The observed detection limit and sensitivity Tyrosine-protein kinase BLK for PtCu NC electrode was 5 μM and 295.3 μA mM-1 cm-2, respectively, with a wide linear range from 5 μM to 22.25 mM. On the basis of our research, the PtCu NC

electrode has potential applications for the design of hydrogen peroxide sensor. Acknowledgements This study is supported by the National Natural Science Foundation of China (21101136), Foundation of Scientific and Technological Research Program of Chongqing Municipal Education Commission (grant no. KJ121213), Chongqing Natural Science Foundation (cstc2013jcyjA20023), Talent Introduction Foundation of Chongqing University of Arts and Sciences (R2012cl14, R2013CJ05), Foundation of Chongqing University of Arts and Sciences (Z2011XC15, Z2013CJ01), and Graphene Research Project of Research Center for Materials Interdisciplinary Science. References 1. Lian W, Wang L, Song Y, Yuan H, Zhao S, Li P, Chen L: A hydrogen peroxide sensor based on electrochemically roughened silver electrodes. Electrochim Acta 2009, 54:4334–4339.CrossRef 2. Wang MY, Shen T, Wang M, Zhang D, Chen J: One-pot green synthesis of Ag nanoparticles-decorated reduced graphene oxide for efficient nonenzymatic H 2 O 2 biosensor. Mater Lett 2013, 107:311–314.