2, Nav1.3 (Black et al., 1995a), Nav1.5 (Black et al., 1998), and Nav1.6 (Reese and Caldwell, 1999), and microglia have been shown to express Nav1.5 and Nav1.6 (Craner et al., 2005 and Black et al., 2009). Human red blood cells have been shown to express Nav1.4 and Nav1.7 (Hoffman et al., 2004). Although this article focuses on the Nav1.1–Nav1.9 pore-forming α-subunits of sodium channels, there is also evidence demonstrating the presence of AZD9291 cost sodium channel β-subunits in some nonexcitable cells (Oh and Waxman, 1994), where they are postulated to function as cell-adhesion molecules (Brackenbury et al.,

2010). As described below, voltage-gated sodium channels contribute to diverse effector functions of nonexcitable cells. This is all the more remarkable because, in general, estimated densities of sodium channels in nonexcitable cells are substantially lower than those in excitable cells (<1 versus 2–75 μm−2, respectively; see, e.g., Sontheimer et al., 1992). Spinal cord astrocytes in vitro are an exception and can express sodium

channels at densities estimated to be as high as 10 channels per μm2 (Sontheimer et al., 1992), which, although not supporting action potential electrogenesis close to resting potential, can support the production of all-or-none overshooting action-potential-like responses when the cells are hyperpolarized to levels that remove resting inactivation (Sontheimer and Waxman, 1992). The density of sodium channels in cells such as astrocytes in vitro depends DAPT cell line on the milieu to which the cells are exposed (Thio and Sontheimer, PTPRJ 1993 and Thio et al., 1993), raising the question of whether channel expression is an artifact of culture. Importantly, however, astrocytes within slices of hippocampus, cerebral cortex, spinal cord, and cerebellum also express sodium currents (Sontheimer and Waxman, 1993, Chvátal et al., 1995 and Bordey and Sontheimer, 2000). Thus, expression of these

channels within astrocytes can occur within a relatively normal milieu and is not an artifact of culture. Further confirmation of this comes from immunocytochemical studies that have demonstrated the expression of sodium channels in astrocytes in situ within both the rodent (Black et al., 1994 and Black et al., 1998) and the human (Black et al., 2010) brain. The expression of sodium channels in nonexcitable cells is not static and, on the contrary, may change markedly depending on the developmental and/or physiological state of the cells. For instance, differentiation of cells of the oligodendrocyte lineage, from oligodendrocyte precursor cells (OPCs) to mature myelinating oligodendrocytes, is accompanied by switches in patterns of phenotypic expression (see Levine et al., 2001), including the downregulation of sodium channels (Paez et al., 2009). OPCs express robust voltage-sensitive sodium currents.