, 2005). Surprisingly, S. pyogenes protein Prp does not interact with plasminogen and plasmin via lysine, however only via arginine and histidine residues (Sanderson-Smith et al., 2007). GBS bind plasminogen only by the glyceraldehyde-3-phosphate dehydrogenase (Seifert et al., 2003). Matrix metalloproteinases/metalloproteases (MMPs) are zinc- or cobalt-dependent enzymes that play a crucial role in normal function and development of CNS. This large group includes collagenases, gelatinases, stromelysins, matrilysin, membrane-type metalloproteinases, and metalloelastases. MMPs differ in cellular sources and substrate specificity, but structural domains remain the same (Kieseier et al.,

1999). MMPs may alter inflammatory cytokine activity, cleave cell surface receptors, AZD6244 activate caspase-3, and Selleck Forskolin regulate other MMP family members (Kawasaki et al., 2008). Together with serine and cysteine proteases, they are able to degenerate and remodulate connective tissues. This damage leads to extravasation of blood-borne proteins, formation of brain edema, and neuronal damage. Pathogens exploit this extravasation to cross various barriers including BBB. Basal level of MMP expression in the brain is low; however, during infections, basal level of MMP expression elevates markedly. MMPs are expressed by most of the resident CNS cells such as ECs, astrocytes, microglia, and neurons together with the infiltrating immune cells (Hummel et al., 2001). Infection of

BMECs with neurotropic viruses

has been connected with decrease and/or redistribution of TJ proteins (Luabeya et al., 2000). MMP activity is highly increased in HIV-infected cells migrating into CNS. Human neuronal and glial cells infected with this virus have been shown to produce large amounts of MMP-2 (Chong et al., 1998). During the WNV infection, it has been observed that inflammatory cytokines, such as TNF-α, macrophage migration inhibitory factor, and MMP-9 play an essential Ergoloid role in BBB disruption (Wang et al., 2004; Arjona et al., 2007). It is likely that activation of MMP-9 in WNV-infected astrocytes is via MMP-3 (Verma et al., 2010). MMPs also play an important role in bacterial meningitis. In fact, MMP-8 and MMP-9, but not MMP-2 and MMP-3, are upregulated in CSF during the meningitis caused by H. influenzae, N. meningitidis, and S. pneumoniae (Leppert et al., 2000). Treponema denticola (Gaibani et al., 2010) and cell wall of Streptococcus suis strongly stimulate the production of MMP-9, whereas zinc metalloproteinase ZmpC of S. pneumoniae cleaves human MMP-9 into its active form (Oggioni et al., 2003), which leads to the BBB disruption (Jobin et al., 2006). MMP-8 is also associated with tissue destruction during Streptococcus sanguinis, N. meningitidis, and Fusobacterium nuclearum infections (Shin et al., 2008; Schubert-Unkmeir et al., 2010). Tissue destruction by N. meningitidis is a consequence of proteolysis of TJ protein occludin by MMP-8.