After co-culture with CMV-infected MRC-5, NK cells remained negative for KIR2DL1 and KIR2DL3, demonstrating that the increase in expression of the respective KIR was most likely due to expansion of KIR+ NK cells rather than induction of KIR expression in KIR− NK cells (data not shown).

As KIR3DS1 expression is detectable only barely above background staining on primary NK cells [20], flow cytometric sorting of KIR3DS1+ from KIR3DS1− cells was not possible, and formal proof that the increase in KIR3DS1 detected after exposure Proteases inhibitor to CMV is still lacking. To exclude the possibility that changes in KIR repertoire were induced by the presence of B- and T lymphocytes, we cultured FACS-sorted NK cells from CMV-seropositive donors in the presence of MRC-5 with and without CMV. Changes in the KIR repertoire were EPZ-6438 molecular weight closely recapitulated by those found if PBMCs were co-cultured from the same donors, showing that the specific expansion could not be ascribed to the presence of lymphocytes other than NK cells in the co-culture assay (Supporting Information Fig. 3). In order to assess how NK cells respond functionally to exposure to CMV infected target cells, we assessed CD107a expression as a marker of degranulation and IFN-γ production by intracellular cytokine staining. After two and 3 weeks of culture, all NK-cell subsets of CMV-seropositive and

-seronegative donors exposed to CMV in vitro degranulated and produced IFN-γ at the level of positive controls (PMA), suggesting nonspecific activation (data not shown). When analyzed earlier, we detected a significant increase in degranulation and IFN-γ production in CMV-exposed NK cells already at 3 days of co-culture. Extending previous results, degranulation

and cytokine production were stronger in CMV-seropositive than in CMV-seronegative donors, and were significantly higher for the HLA-C binding KIR2DL1 than for the HLA-B binding KIR3DL1 (Fig. 5). This analysis of the impact of previous infection with CMV on the KIR repertoire of NK cells was prompted by the observation that transplant recipients are relatively protected from CMV replication if they carried B-haplotype associated activating KIR genes [5-8]. In our most recent analysis, protective effects were most evident in Y-27632 2HCl carriers of activating KIR genes located in the telomeric part of the KIR haplotype [6]. This part of the KIR gene cluster contains the activating receptors KIR2DS1, KIR3DS1, and KIR2DS5. The strong linkage disequilibrium between these genes makes it unlikely that population-based genetic association studies will be helpful in further identifying the resistance locus [21]. We therefore aimed in this study to analyze if previous infection with CMV alters the repertoire of KIR expression both in freshly isolated cells as well as after exposure to CMV in an in vitro co-culture model.