It is conjectured that the disintegration is due to the stronger stacking interactions between the benzene ring on the surface of PS and SWNHs than that between SWNHs aggregates. Because SWNHs particles were unstable coated on PS surface, partial SWNHs particles on PS surface diffused to water droplet and suspended by buoyancy of water. Then a new SWNHs/PS surface with less SWNHs particles than original SWNHs/PS

surface was formed, as a result, the hydrophobicity of the surface was lowered and it resulted in decrease of the contact angle (Additional file 1: Figure S5). SWNHs inhibited mitotic entry of N9 cells, especially in pre-treated with LPS To assure how the SWNHs affect cellular mitosis, we incorporated BrdU into the control. We found that the accumulation Romidepsin price of mitotic N9 cells pre-treated with or without learn more LPS were CYC202 in vivo significantly delayed by SWNHs at every time point followed with the increasing

concentrations of SWNHs (P < 0.01). The accumulation of mitotic N9 cells pre-treated with LPS (Figure 1B) was much more than that without LPS (Figure 1A). Figure 1 SWNHs inhibited mitotic entry of N9 cells, especially in pre-treated with LPS. To assure how the SWNHs affect cellular mitosis, we incorporated BrdU into the control. We found that the accumulation of mitotic N9 cells pre-treated with or without LPS was significantly delayed by SWNHs at every time point followed with the increasing concentrations of SWNHs (P < 0.01), and the accumulation of mitotic N9

cells pre-treated with LPS (B) were much more than that Branched chain aminotransferase without LPS (A). The mitotic entry of N9 cells pre-treated with LPS (D) was more than N9 cells (C). SWNHs inhibited mitotic entry of N9 cells pre-treated with or without LPS significantly at every time point followed with the increasing concentrations of SWNHs (P < 0.01). The mitotic entry inhibited by SWNHs in N9 cells pre-treated with LPS (D) was more significant than N9 cells (C). All data are represented as mean ± SEM. The mitotic entry of N9 cells pre-treated with LPS (Figure 1D) was more than N9 cells (Figure 1C). SWNHs inhibited mitotic entry of N9 cells pre-treated with or without LPS significantly at every time point followed with the increasing concentrations of SWNHs (P <0.01). The mitotic entry inhibited by SWNHs in N9 cells pre-treated with LPS (Figure 1D) was more significant than N9 cells (Figure 1C). SWNHs inhibited growth and proliferation of N9 cells, especially in pre-treated with LPS By XTT assays, we investigated the effect of SWNHs on cell growth and found that the growth of N9 cells pre-treated with LPS (Figure 2B) was much more significant than that in N9 cells (Figure 2A). The growth of cells was significantly inhibited by SWNHs at each time point in a dose-dependent manner (P < 0.001), especially in cells pre-treated with LPS (Figure 2B). Figure 2 SWNHs inhibited growth and proliferation of N9 cells, especially in pre-treated with LPS.