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“Background Methicillin-resistant staphylococci represent a great challenge for treatment and public health. In staphylococci, methicillin resistance is mainly due to the expression of the mecA gene, which specifies penicillin binding protein 2a (PBP2a), a transpeptidase with a low affinity for β-lactams [1, 2]. mecA is carried by a mobile genetic element (MGE) termed the staphylococcal cassette chromosome mec

(SCCmec) [2, 3]. Generally, SCCmec contains two essential components, i.e. the mec gene complex and the ccr gene complex. The mec gene complex consists of mecA, the regulatory genes and associated insertion sequences and has been classified into six different classes, i.e. A, B, C1, C2, D and E. Cassette chromosome recombinase (ccr) genes (ccrC or the pair of ccrA and ccrB) encode recombinases mediating integration and excision of SCCmec into and from the chromosome [2, 3]. The ccr gene(s) find more and surrounding genes form the ccr gene complex. A Staphylococcus haemolyticus clinical isolate, WCH1, was found carrying mecA but no ccr genes. Although clinical isolates of S. haemolyticus containing mecA but lacking ccr genes have been reported previously [4–6], information about the detailed contexts of mecA is largely absent. The genetic context of mecA in WCH1 was therefore investigated using long-range PCR, PCR mapping, inverse PCR and sequencing as described previously [7]. Results and discussion The minimum inhibitory concentration (MIC) of cefoxitin against WCH1 was 128 μg/ml.