pleuropneumoniae adherence to eukaryotic cells. Further, the autotransporter adhesin

of Bordetella pertussis, pertactin, has been used as a component of the commercial multivalent vaccine (Miller, 1999; Jefferson et al., 2003). Therefore, we also presumed that the autotransporter adhesin may serve as a novel potential vaccine candidate for the multivalent vaccine for A. pleuropneumoniae infection; this aspect needs to be studied further. Additionally, the diverse distribution of the 19 differential gene sequences among the 15 selleckchem serotypes will contribute to the development of serotyping methods for A. pleuropneumoniae. Multiplex PCRs for the simultaneous identification of serotypes 2, 5, and 6 (Jessing et al., 2003), serotypes 1, 2, and 8(Schuchert et al., 2004), serotypes 1, 7, and 12 (Angen et al., 2008),

and serotypes 3, 6, and 8 (Zhou et al., 2008) have been published. In our study, two differential DNA sequences –tbpB1 (a6) and tbpB2 (a23) – were present only in serotypes 1, 6, 12, and 14, and see more this finding raises the possibility of a multiplex PCR method that can distinguish between serotypes 1, 6, 12, and 14 using specific primers for these serotypes. Similarly, five differential DNA sequences, namely, wzy (b12), rfaG (b13), glf1 (b15), glf2 (b16), and pst (b17), were present only in serotypes 3, 6, 8, and 15, thereby indicating the possibility of a multiplex PCR method in which the serotypes 3, 6, 8, and 15 can be distinguished using specific primers. There are two subtypes of

A. pleuropneumoniae serotype 1 (1a and 1b). A previous study suggested that pigs immunized with subtype 1a were better protected against challenge with 1a and 1b, in comparison with pigs vaccinated with 1b and challenged with 1a and 1b (Jolie et al., 1995). Therefore, we initially selected A. pleuropneumoniae strains CVCC259 (serotype 1a) and CVCC261 (serotype 3) as the study subjects. However, the genomic differences of the A. pleuropneumoniae serotypes Protein tyrosine phosphatase 1b and 3 need to be studied further. In conclusion, the 19 differential genes are not only diagnostic targets but also potential candidates for an A. pleuropneumoniae multivalent vaccine. Further investigations into the role of these genes are in progress. We expect that the characterization of these genes in the serotypes of A. pleuropneumoniae will guide future research on the pathogenic mechanisms of A. pleuropneumoniae and the development of multivalent vaccines for A. pleuropneumoniae infections. This work was supported by grants from the Special Purpose Scientific Research of Doctor Subject Foundation of Chinese Ministry of Education (no. 20060183054) and the National Natural Science Foundation of China (no. 30870089). L.L. and W.H. contributed equally to this work. “
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