The balance of inflammation, innate immunity and adaptive immunity interfacing with the complex commensal biofilms, controlling pathogens that emerge in the biofilms, minimizing Bortezomib manufacturer local collateral tissue damage from chronic inflammation and down-regulating systemic responses to the infections remain ill-defined. The commensal opportunistic pathogens provoke both a localized and systemic response during the disease [39–41], with systemic inflammatory responses being generally low in individuals with a healthy periodontium or in subjects with reversible gingival inflammation (i.e. gingivitis) and increasing in periodontitis

patients [40,42]. Thus, an interaction between the systemic responses to periodontitis and the changes that occur during pregnancy could be predicted to increase the risk of adverse pregnancy outcomes [43–45]. The objectives of this study were to document profiles of various systemic inflammatory mediators in female baboons during their pregnancy resulting from ligature-induced periodontitis. The targeted mediators would be those that could contribute to adverse pregnancy outcomes and might be predictive of the biological risk linking periodontal disease with these events. These data should contribute to the development of a pathway that explores the contribution Silmitasertib of oral infection and systemic host responses to birth outcomes using a non-human primate model. An experimental cohort of 288 Papio anubis (168 experimental; 120

controls) were examined in this study. Inclusion in the study is dependent upon the following criteria: (i) dams must have a minimum of 20 teeth; (ii) be in good general health based upon an examination by the veterinarian; (iii) range in age from 6–13 years; and (iv) have produced previous offspring. Mothers were excluded if they demonstrated systemic illness that required veterinary

treatment during the course Dolichyl-phosphate-mannose-protein mannosyltransferase of the project that would adversely impact the pregnancy outcome (i.e. infection) and/or administration of antibiotic and/or anti-inflammatory therapy, which could confound the onset and severity of periodontitis. Loss of body weight ≥15% also excluded the baboon from further participation in this project. Nulliparous dams (e.g. previous births increase likelihood of successful breeding for this study), dams of extreme ages, either younger or older, and those dams having fewer than 20 teeth were excluded. The animals were sampled prospectively at three time-points during the study. The study design has been described previously [46]; briefly, however, the experimental animals were sampled at baseline (clinical examination, serum) and teeth in quadrants one and four were ligated. A second sampling took place at mid-gestation (∼3 months) into the pregnancy and ligatures were tied on the contralateral maxillary and mandibular quadrants (quadrants two and three). The third sample was obtained from 2 to 10 days after delivery and the ligatures were removed.