tRNA are the preferred sites for host integration

of Smp131 and the related phages: tRNA-Thr for Smp131 and prophage of S. maltophilia K279a; tRNA-Lys for prophages of X. campestris pv. campestris ATCC33913, X. oryzae pv. oryzae strains MAFF311018, and KACC10331; and tRNA-Asn for prophage of X. oryzae pv. oryzae PXO99A and remnant of X. axonopodis pv. citri 306. Regions flanking the prophages are varied highly in nucleotide sequence and rich in transposase genes, suggesting that frequent insertion/excision had occurred. Conclusions: Prevalence of closely related prophages in Stenotrophomonas and Xanthomonads may have contributed to the diversity of these closely related species owing to possible horizontal gene CHIR98014 molecular weight transfer mediated by the phages.”
“We investigated linear aliphatic dialkynes Taselisib inhibitor as a new structural class of i,i+7 linkers for the double-click stapling of p53-based peptides. The optimal combination of azido amino acids and dialkynyl linker length for MDM2 binding was determined. In a direct comparison between aliphatic

and aromatic staple scaffolds, the aliphatic staples resulted in superior binding to MDM2 in vitro and superior p53-activating capability in cells when using a diazidopeptide derived from phage display. This work demonstrates that the nature of the staple scaffold is an important factor that can affect peptide bioactivity find more in cells.”
“The influence of different light sources and light intensity on mycophenolic acid (MPA) by Penicillium brevicompactum in batch culture was significant and demonstrated in this study.

MPA batch cultures under various light sources including fluorescent light, red LED light (630 nm) and blue LED light (470 nm), were investigated in a light controlled photo-bioreactor. Results of cultures under fluorescent light indicated that optimal maximum cell density and maximum MPA concentration (P(max)) achieved at 1400 lux were 9.40 and 0.48 g L(-1), respectively. Results of culture under LED lights indicated that the optimal culture condition for cell growth was red LED light at 600 lux with cell yield, 0.88 g g(-1), while that for MPA formation was blue LED light at 600 lux with specific product yield, 0.056 g g(-1). Red LED light stimulated both cell growth and MPA formation; however, blue LED light inhibited cell growth but stimulated MPA formation. A novel two-stage LED light process has been successfully demonstrated to optimize MPA fermentation of P. brevicompactum, and the highest P(max) of 0.56 g L(-1) was achieved with 87% improvement as compared with that of the batch in the dark. (C) 2010 Elsevier Inc. All rights reserved.